Monday, 18 February 2013

Lab: Tests For Biochemical Molecules


This is my lab for the tests for biochemical molecules.

First we did the test for fats. We rubbed a piece of cupcake, cheese, apple, lettuce, tomato, bread, butter, orange and pancake on a piece of recycled paper. After a couple of minutes, the ones that consisted of fats left an oily residue behind. Here are some before and after pictures.


Next we did the test for starch. We used a spot plate and a couple of drops of Iodine to determine which ones were positive. We were able to figure that out because a dark purple colour indicates starch is present. Here are some pictures from before and after Iodine solution was added.



*Note: the foods circled on the chart in the photo tested positive.
Our last test was for carbohydrates. We used test tubes filled with water and pieces of the various foods inside a hot bath. We added Benedict solution and waited for two minutes. The ones that turned an orange colour indicated single sugars are present. Here are also some photos.




This is a chart I made that shows the positive and negative items for each category.


Items
Fats
Starch
Carbohydrates
Cupcake
Positive
Positive
Positive
Cheese
Positive
Negative
Positive
Apple
Negative
Negative
Positive
Lettuce
Negative
Negative
Not Tested
Tomato
Negative
Positive
Not Tested
Bread
Negative
Positive
Positive
Butter
Positive
Negative
Negative
Orange
Negative
Negative
Positive
Pancake
Negative
Positive
Not Tested

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